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Saturday, May 25, 2013

Daidzein sulfoconjugates are potent inhibitors of sterol sulfatase (EC 3.1.6.2)

Posted by Chantel Martiromo. research contributed by PubMed

Recent studies have associated high dietary isoflavone intake with low incidence of breast cancer. In the study to investigate the effects of the isoflavone daidzein and its sulfoconjugates, daidzein-4'-O-sulfate and daidzein-7,4'-di-O-sulfate, on sterol sulfatase acitivity using dehydroepiandrosterone sulfate as substrate, posted in PubMed, showed that while daidzein does not affect sterol sulfatase, its sulfoconjugates are potent inhibitors of this enzyme. Kinetic analyses reveal that daidzein-4'-O-sulfate and daidzein-7,4'-di-O-sulfate inhibit sterol sulfatase competitively with respect to the steroid substrate and with Ki values of 5.9 and 1 microM, respectively. Daidzein sulfo-conjugates also inhibit hydroxysteroid and phenol sulfotransferases but at much higher concentrations.


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